› 2axc

X-ray diffraction
1.7Å resolution

Crystal structure of ColE7 translocation domain

Released:
DOI: 10.2210/pdb2axc/pdb
PDB entry 2axc coloured by chain, front view.
PDB entry 2axc coloured by chain, side view.
PDB entry 2axc coloured by chain, top view.
Source organism: Escherichia coli str. K-12 substr. W3110
Primary publication:
High-resolution crystal structure of a truncated ColE7 translocation domain: implications for colicin transport across membranes.
Cheng YS, Shi Z, Doudeva LG, Yang WZ, Chak KF, Yuan HS
J Mol Biol 356 22-31 (2006)
PMID: 16360169

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175219 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Colicin-E7 Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 27.59 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47112 (Residues: 60-316; Coverage: 45%)
Gene names: cea, colE7
Sequence domains: Colicin-like bacteriocin tRNase domain
Structure domains: Single helix bin

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P3121
Unit cell:
a: 59.038Å b: 59.038Å c: 132.178Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.181 0.208
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Colicin biology.
Cascales et al. (2007)
9 other articles

2 mentions without citation

Conservation of complex knotting and slipknotting patterns in proteins.
SuĊ‚kowska et al. (2012)
1 more