› 2axd

Solution NMR

solution structure of the theta subunit of escherichia coli DNA polymerase III in complex with the epsilon subunit

Released:
DOI: 10.2210/pdb2axd/pdb
PDB entry 2axd coloured by chain, ensemble of 12 models, front view.
PDB entry 2axd coloured by chain, ensemble of 12 models, side view.
PDB entry 2axd coloured by chain, ensemble of 12 models, top view.
Source organism: Escherichia coli
Primary publication:
Structure of the theta subunit of Escherichia coli DNA polymerase III in complex with the epsilon subunit.
Keniry MA, Park AY, Owen EA, Hamdan SM, Pintacuda G, Otting G, Dixon NE
J Bacteriol 188 4464-73 (2006)
PMID: 16740953

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142204 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase III subunit theta Chain: S
Molecule details ›
Chain: S
Length: 76 amino acids
Theoretical weight: 8.86 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0ABS8 (Residues: 1-76; Coverage: 100%)
Gene names: JW1831, b1842, holE
Sequence domains: DNA polymerase III, theta subunit
Structure domains: DNA polymerase III-theta

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Protein NMR using paramagnetic ions.
Otting G. (2010)
9 more

3 mentions without citation

Protein-protein HADDocking using exclusively pseudocontact shifts.
Schmitz et al. (2011)
2 more