› 2ayb

X-ray diffraction
3.2Å resolution

Crystal structure of HPV6a E2 DNA Binding Domain bound to a 16 base pair DNA target

Released:
DOI: 10.2210/pdb2ayb/pdb
PDB entry 2ayb coloured by chain, front view.
PDB entry 2ayb coloured by chain, side view.
PDB entry 2ayb coloured by chain, top view.
Source organism: Human papillomavirus type 6a
Primary publication:
The recognition of local DNA conformation by the human papillomavirus type 6 E2 protein.
Hooley E, Fairweather V, Clarke AR, Gaston K, Brady RL
Nucleic Acids Res 34 3897-908 (2006)
PMID: 16914454

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115095 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Regulatory protein E2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 87 amino acids
Theoretical weight: 10.24 KDa
Source organism: Human papillomavirus type 6a
Expression system: Escherichia coli
UniProt:
  • Canonical: Q84294 (Residues: 282-368; Coverage: 24%)
Gene name: E2
Sequence domains: E2 (early) protein, C terminal
Structure domains: Alpha-Beta Plaits
5'-D(*CP*AP*AP*CP*CP*GP*AP*AP*TP*TP*CP*GP*GP*TP*TP*G)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Human papillomavirus type 6a
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER X8 PROTEUM
Spacegroup: P61
Unit cell:
a: 75.247Å b: 75.247Å c: 97.426Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.189 0.297
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 citation in other articles

Comprehensive comparison of the interaction of the E2 master regulator with its cognate target DNA sites in 73 human papillomavirus types by sequence statistics.
Sánchez et al. (2008)
10 more

4 mentions without citation

The papillomavirus E2 proteins.
McBride AA. (2013)
3 more