› 2aze

X-ray diffraction
2.55Å resolution

Structure of the Rb C-terminal domain bound to an E2F1-DP1 heterodimer

Released:
DOI: 10.2210/pdb2aze/pdb
PDB entry 2aze coloured by chain, front view.
PDB entry 2aze coloured by chain, side view.
PDB entry 2aze coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the Rb C-terminal domain bound to E2F1-DP1: a mechanism for phosphorylation-induced E2F release.
Rubin SM, Gall AL, Zheng N, Pavletich NP
Cell 123 1093-106 (2005)
PMID: 16360038

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-139031 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Transcription factor Dp-1 Chain: A
Molecule details ›
Chain: A
Length: 155 amino acids
Theoretical weight: 17.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14186 (Residues: 199-350; Coverage: 37%)
Gene names: DP1, TFDP1
Sequence domains: Transcription factor DP
Structure domains: Transcription factor DP
Transcription factor E2F1 Chain: B
Molecule details ›
Chain: B
Length: 106 amino acids
Theoretical weight: 11.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q01094 (Residues: 200-301; Coverage: 23%)
Gene names: E2F1, RBBP3
Sequence domains: E2F transcription factor CC-MB domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Retinoblastoma-associated protein Chain: C
Molecule details ›
Chain: C
Length: 46 amino acids
Theoretical weight: 5.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06400 (Residues: 829-874; Coverage: 5%)
Gene name: RB1
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C, NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 146.8Å b: 168.6Å c: 48.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.262
Expression system: Escherichia coli

Quick links

Citations

32 review citations

Emerging biomarkers in head and neck cancer in the era of genomics.
Kang et al. (2015)
31 more

23 mentions without citation

Molecular mechanisms underlying RB protein function.
Dick et al. (2013)
22 more