› 2azl

X-ray diffraction
2.8Å resolution

Crystal structure for the mutant F117E of Thermotoga maritima octaprenyl pyrophosphate synthase

Released:
DOI: 10.2210/pdb2azl/pdb
PDB entry 2azl coloured by chain, front view.
PDB entry 2azl coloured by chain, side view.
PDB entry 2azl coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Homodimeric hexaprenyl pyrophosphate synthase from the thermoacidophilic crenarchaeon Sulfolobus solfataricus displays asymmetric subunit structures.
Sun HY, Ko TP, Kuo CJ, Guo RT, Chou CC, Liang PH, Wang AH
J Bacteriol 187 8137-48 (2005)
PMID: 16291686

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-194820 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Octoprenyl-diphosphate synthase, putative Chain: A
Molecule details ›
Chain: A
Length: 299 amino acids
Theoretical weight: 33.88 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X1M1 (Residues: 1-299; Coverage: 100%)
Gene name: TM_1535
Sequence domains: Polyprenyl synthetase
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: I422
Unit cell:
a: 151.514Å b: 151.514Å c: 64.606Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.276
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Structural and Chemical Biology of Terpenoid Cyclases.
Christianson DW. (2017)
2 more

1 mention without citation

Homodimeric hexaprenyl pyrophosphate synthase from the thermoacidophilic crenarchaeon Sulfolobus solfataricus displays asymmetric subunit structures.
Sun et al. (2005)