› 2b0c

X-ray diffraction
2Å resolution

The crystal structure of the putative phosphatase from Escherichia coli

Released:
DOI: 10.2210/pdb2b0c/pdb
PDB entry 2b0c coloured by chain, front view.
PDB entry 2b0c coloured by chain, side view.
PDB entry 2b0c coloured by chain, top view.
Source organism: Escherichia coli K-12
Entry authors: Zhang R, Skarina T, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 1-phosphate + H(2)O = D-glucose + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141753 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-D-glucose 1-phosphate phosphatase YihX Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 23.58 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8Y3 (Residues: 1-199; Coverage: 100%)
Gene names: JW5566, b3885, yihX
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand G1P 1 x G1P
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 62.256Å b: 62.256Å c: 127.038Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.201 0.259
Expression system: Escherichia coli BL21(DE3)

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