› 2b0l

X-ray diffraction
2.9Å resolution

C-terminal DNA binding domain of transcriptional pleiotropic repressor CodY.

Released:
DOI: 10.2210/pdb2b0l/pdb
PDB entry 2b0l coloured by chain, front view.
PDB entry 2b0l coloured by chain, side view.
PDB entry 2b0l coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The structure of CodY, a GTP- and isoleucine-responsive regulator of stationary phase and virulence in gram-positive bacteria.
Levdikov VM, Blagova E, Joseph P, Sonenshein AL, Wilkinson AJ
J Biol Chem 281 11366-73 (2006)
PMID: 16488888

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153949 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Global transcriptional regulator CodY Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 102 amino acids
Theoretical weight: 11.29 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P39779 (Residues: 168-259; Coverage: 36%)
Gene names: BSU16170, codY
Sequence domains: CodY helix-turn-helix domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200, ESRF BEAMLINE ID23-1
Spacegroup: P422
Unit cell:
a: 68.128Å b: 68.128Å c: 164.903Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.25
Expression system: Escherichia coli BL21

Quick links

Citations

8 review citations

Amino acids and mTORC1: from lysosomes to disease.
Efeyan et al. (2012)
7 more

2 mentions without citation

Diversity in (p)ppGpp metabolism and effectors.
Liu et al. (2015)
1 more