› 2b0v

X-ray diffraction
1.55Å resolution

NUDIX hydrolase from Nitrosomonas europaea.

Released:
DOI: 10.2210/pdb2b0v/pdb
PDB entry 2b0v coloured by chain, front view.
PDB entry 2b0v coloured by chain, side view.
PDB entry 2b0v coloured by chain, top view.
Source organism: Nitrosomonas europaea
Entry authors: Osipiuk J, Skarina T, Savchenko A, Edwards A, Joachimiak A, MCSG, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-182721 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatase NudJ Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 17.44 KDa
Source organism: Nitrosomonas europaea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82XR9 (Residues: 1-149; Coverage: 100%)
Gene names: NE0184, nudJ
Sequence domains: NUDIX domain
Structure domains: Nucleoside Triphosphate Pyrophosphohydrolase

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand NA 1 x NA
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 55.45Å b: 66.509Å c: 73.812Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.167 0.206
Expression system: Escherichia coli BL21(DE3)

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