› 2b18

X-ray diffraction
1.8Å resolution

N-terminal GAF domain of transcriptional pleiotropic repressor CodY.

Released:
DOI: 10.2210/pdb2b18/pdb
PDB entry 2b18 coloured by chain, front view.
PDB entry 2b18 coloured by chain, side view.
PDB entry 2b18 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
The structure of CodY, a GTP- and isoleucine-responsive regulator of stationary phase and virulence in gram-positive bacteria.
Levdikov VM, Blagova E, Joseph P, Sonenshein AL, Wilkinson AJ
J Biol Chem 281 11366-73 (2006)
PMID: 16488888

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153948 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Global transcriptional regulator CodY Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.55 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P39779 (Residues: 1-155; Coverage: 60%)
Gene names: BSU16170, codY
Sequence domains: CodY GAF-like domain
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:
Ligand ILE 1 x ILE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX10.1
Spacegroup: P21212
Unit cell:
a: 34.696Å b: 86.081Å c: 54.819Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.212
Expression system: Escherichia coli BL21

Quick links

Citations

9 review citations

Amino acids and mTORC1: from lysosomes to disease.
Efeyan et al. (2012)
8 more

5 mentions without citation

Structural rearrangement accompanying ligand binding in the GAF domain of CodY from Bacillus subtilis.
Levdikov et al. (2009)
4 more