› 2b1c

X-ray diffraction
2.2Å resolution

5'-D(*GP*CP*GP*TP*GP*GP*GP*AP*CP*C)-3' Zif268 binding site

Released:
DOI: 10.2210/pdb2b1c/pdb
PDB entry 2b1c coloured by chain, front view.
PDB entry 2b1c coloured by chain, side view.
PDB entry 2b1c coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Signatures of protein-DNA recognition in free DNA binding sites.
Locasale JW, Napoli AA, Chen S, Berman HM, Lawson CL
J Mol Biol 386 1054-65 (2009)
PMID: 19244617

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115101 (preferred)
Entry contents:
2 distinct DNA molecules
Macromolecules (2 distinct):
5'-D(*GP*CP*GP*TP*GP*GP*GP*AP*CP*C)-3' Chain: A
Molecule details ›
Chain: A
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: Synthetic construct
Expression system: Not provided
5'-D(*GP*GP*TP*CP*CP*CP*AP*CP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.01 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P6122
Unit cell:
a: 38.482Å b: 38.482Å c: 78.225Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.234 0.286
Expression system: Not provided

Quick links

Citations

1 review citation

DNA dynamics and single-molecule biology.
Duzdevich et al. (2014)
21 other articles

2 mentions without citation

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
1 more