› 2b1n

X-ray diffraction
2.4Å resolution

Crystal structure of a papain-fold protein without the catalytic cysteine from seeds of Pachyrhizus erosus

Released:
DOI: 10.2210/pdb2b1n/pdb
PDB entry 2b1n coloured by chain, front view.
PDB entry 2b1n coloured by chain, side view.
PDB entry 2b1n coloured by chain, top view.
Source organism: Pachyrhizus erosus
Primary publication:
Crystal structure of a papain-fold protein without the catalytic residue: a novel member in the cysteine proteinase family.
Zhang M, Wei Z, Chang S, Teng M, Gong W
J Mol Biol 358 97-105 (2006)
PMID: 16497323

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162641 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Peptidase C1A papain C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.12 KDa
Source organism: Pachyrhizus erosus
UniProt:
  • Canonical: Q3Y6U7 (Residues: 1-246; Coverage: 100%)
Gene name: spe31
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases
peptide (LYS)(ALA)(SER)(VAL)(GLY) Chain: B
Molecule details ›
Chain: B
Length: 5 amino acids
Theoretical weight: 462 Da
Source organism: Pachyrhizus erosus
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41212
Unit cell:
a: 61.358Å b: 61.358Å c: 143.988Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.267
Expression system: Not provided

Quick links

Citations

1 review citation

Production of plant proteases in vivo and in vitro--a review.
González-Rábade et al. (2011)
4 other articles

1 mention without citation

The origin and functional transition of P34.
Li et al. (2013)