› 2b25

X-ray diffraction
2.5Å resolution

Human putative tRNA(1-methyladenosine)methyltransferase

Released:
DOI: 10.2210/pdb2b25/pdb
PDB entry 2b25 coloured by chain, front view.
PDB entry 2b25 coloured by chain, side view.
PDB entry 2b25 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Tempel W, Wu H, Dong A, Zeng H, Loppnau P, Arrowsmith C, Edwards A, Sundstrom M, Weigelt J, Bochkarev A, Plotnikov AN, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine(58) in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(58) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-189768 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (adenine(58)-N(1))-methyltransferase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 336 amino acids
Theoretical weight: 37.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9BVS5 (Residues: 144-477; Coverage: 70%)
Gene name: TRMT61B
Sequence domains: tRNA methyltransferase complex GCD14 subunit
Structure domains:

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
1 bound ligand:
Ligand UNX 4 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: C2221
Unit cell:
a: 75.533Å b: 157.106Å c: 113.492Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.247 0.282
Expression system: Escherichia coli BL21(DE3)

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