› 2b2h

X-ray diffraction
1.54Å resolution

Ammonium Transporter Amt-1 from A. fulgidus (AS)

Released:
DOI: 10.2210/pdb2b2h/pdb
PDB entry 2b2h coloured by chain, front view.
PDB entry 2b2h coloured by chain, side view.
PDB entry 2b2h coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Crystal structure of the archaeal ammonium transporter Amt-1 from Archaeoglobus fulgidus.
Andrade SL, Dickmanns A, Ficner R, Einsle O
Proc Natl Acad Sci U S A 102 14994-9 (2005)
PMID: 16214888

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128108 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ammonium transporter Amt1 Chain: A
Molecule details ›
Chain: A
Length: 399 amino acids
Theoretical weight: 42.09 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O29285 (Residues: 1-391; Coverage: 100%)
Gene names: AF_0977, amt1
Sequence domains: Ammonium Transporter Family
Structure domains: Ammonium transporter AmtB like domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: R3
Unit cell:
a: 111.408Å b: 111.408Å c: 136.644Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.154 0.154 0.169
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Nitrogen regulation in bacteria and archaea.
Leigh et al. (2007)
27 more

14 mentions without citation

Tolerance to changes in membrane lipid composition as a selected trait of membrane proteins.
Sanders et al. (2011)
13 more