› 2b3d

X-ray diffraction
2.1Å resolution

Crystal structure of Modulator of Drug activity B in complex with flavin adenine dinucleotide

Released:
DOI: 10.2210/pdb2b3d/pdb
PDB entry 2b3d coloured by chain, front view.
PDB entry 2b3d coloured by chain, side view.
PDB entry 2b3d coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Modulator of drug activity B from Escherichia coli: crystal structure of a prokaryotic homologue of DT-diaphorase.
Adams MA, Jia Z
J Mol Biol 359 455-65 (2006)
PMID: 16630630

Function and Biology Details

Reaction catalysed: 
NADPH + a quinone = NADP(+) + a quinol
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-216701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH:quinone oxidoreductase MdaB Chains: A, B
Molecule details ›
Chains: A, B
Length: 204 amino acids
Theoretical weight: 23.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEY5 (Residues: 2-193; Coverage: 100%)
Gene names: JW2996, b3028, mda66, mdaB
Sequence domains: Flavodoxin-like fold
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 48.626Å b: 84.105Å c: 55.49Å
α: 90° β: 110.95° γ: 90°
R-values:
R R work R free
0.2 0.193 0.261
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The diverse antioxidant systems of Helicobacter pylori.
Wang et al. (2006)
4 more

3 mentions without citation

Identification of NAD(P)H quinone oxidoreductase activity in azoreductases from P. aeruginosa: azoreductases and NAD(P)H quinone oxidoreductases belong to the same FMN-dependent superfamily of enzymes.
Ryan et al. (2014)
2 more