› 2b3r

X-ray diffraction
2.3Å resolution

Crystal structure of the C2 domain of class II phosphatidylinositide 3-kinase C2

Released:
DOI: 10.2210/pdb2b3r/pdb
PDB entry 2b3r coloured by chain, front view.
PDB entry 2b3r coloured by chain, side view.
PDB entry 2b3r coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the C2 domain of class II phosphatidylinositide 3-kinase C2alpha.
Liu L, Song X, He D, Komma C, Kita A, Virbasius JV, Huang G, Bellamy HD, Miki K, Czech MP, Zhou GW
J Biol Chem 281 4254-60 (2006)
PMID: 16338929

Function and Biology Details

Reactions catalysed: 
ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-178870 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 15.46 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q61194 (Residues: 1561-1686; Coverage: 8%)
Gene names: Cpk, Pik3c2a
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU, CAMD BEAMLINE GCPCC
Spacegroup: P41212
Unit cell:
a: 53.365Å b: 53.365Å c: 200.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.232
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Phosphatidylinositol 3-phosphates-at the interface between cell signalling and membrane traffic.
Marat et al. (2016)
4 more

3 mentions without citation

A systematic comparative and structural analysis of protein phosphorylation sites based on the mtcPTM database.
Jiménez et al. (2007)
2 more