› 2b44

X-ray diffraction
1.83Å resolution

Truncated S. aureus LytM, P 32 2 1 crystal form

Released:
DOI: 10.2210/pdb2b44/pdb
Model geometry
Fit model/data
PDB entry 2b44 coloured by chain, front view.
PDB entry 2b44 coloured by chain, side view.
PDB entry 2b44 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Crystal structures of active LytM.
Firczuk M, Mucha A, Bochtler M
J Mol Biol 354 578-90 (2005)
PMID: 16269153

Function and Biology Details

Reaction catalysed: 
Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128493 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycyl-glycine endopeptidase LytM Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 14.43 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O33599 (Residues: 185-316; Coverage: 45%)
Gene names: SAOUHSC_00248, lytM
Sequence domains: Peptidase family M23
Structure domains: Glucose Permease (Domain IIA)

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P3221
Unit cell:
a: 100.434Å b: 100.434Å c: 103.038Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.181 0.193
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Bacterial peptidoglycan (murein) hydrolases.
Vollmer et al. (2008)
7 more

9 mentions without citation

Engineering Natural Killer Cells for Cancer Immunotherapy.
Rezvani et al. (2017)
8 more