› 2b4g

X-ray diffraction
1.95Å resolution

Function and Biology Details

Reaction catalysed: 
(S)-dihydroorotate + fumarate = orotate + succinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176527 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydroorotate dehydrogenase (fumarate) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 317 amino acids
Theoretical weight: 34.47 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q57U83 (Residues: 1-313; Coverage: 100%)
Gene name: Tb927.5.3830
Sequence domains: Dihydroorotate dehydrogenase
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 4 x FMN
3 bound ligands:
Ligand BR 11 x BR
Ligand ORO 4 x ORO
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2221
Unit cell:
a: 81.605Å b: 162.63Å c: 163.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.189 0.242
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Structure and organization of mitochondrial respiratory complexes: a new understanding of an old subject.
Lenaz et al. (2010)
7 more

8 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
7 more