› 2b4l

X-ray diffraction
2Å resolution

Crystal structure of the binding protein OpuAC in complex with glycine betaine

Released:
DOI: 10.2210/pdb2b4l/pdb
PDB entry 2b4l coloured by chain, front view.
PDB entry 2b4l coloured by chain, side view.
PDB entry 2b4l coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Molecular determinants for substrate specificity of the ligand-binding protein OpuAC from Bacillus subtilis for the compatible solutes glycine betaine and proline betaine.
Horn C, Sohn-Bösser L, Breed J, Welte W, Schmitt L, Bremer E
J Mol Biol 357 592-606 (2006)
PMID: 16445940

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine betaine-binding protein OpuAC Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 29.78 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46922 (Residues: 26-293; Coverage: 98%)
Gene names: BSU03000, opuAC
Sequence domains: Substrate binding domain of ABC-type glycine betaine transport system
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand BET 1 x BET
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 29.818Å b: 88.486Å c: 95.714Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.255
Expression system: Escherichia coli BL21(DE3)

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Citations

6 review citations

A structural classification of substrate-binding proteins.
Berntsson et al. (2010)
5 more

9 mentions without citation

Characterization of inhibitory and stimulatory forms of the murine natural killer cell receptor 2B4.
Schatzle et al. (1999)
8 more