› 2b4q

X-ray diffraction
2.3Å resolution

Pseudomonas aeruginosa RhlG/NADP active-site complex

Released:
DOI: 10.2210/pdb2b4q/pdb
PDB entry 2b4q coloured by chain, front view.
PDB entry 2b4q coloured by chain, side view.
PDB entry 2b4q coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structure of RhlG, an essential beta-ketoacyl reductase in the rhamnolipid biosynthetic pathway of Pseudomonas aeruginosa.
Miller DJ, Zhang YM, Rock CO, White SW
J Biol Chem 281 18025-32 (2006)
PMID: 16624803

Function and Biology Details

Reaction catalysed: 
(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-193227 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 29.03 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RPT1 (Residues: 1-256; Coverage: 100%)
Gene names: PA3387, rhlG
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM, APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 73.138Å b: 142.61Å c: 95.684Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Quinolones: from antibiotics to autoinducers.
Heeb et al. (2011)
2 more

6 mentions without citation

Effects of Natural Products on Bacterial Communication and Network-Quorum Sensing.
Yang et al. (2020)
5 more