› 2b5a

X-ray diffraction
1.54Å resolution

C.BclI, Control Element of the BclI Restriction-Modification System

Released:
DOI: 10.2210/pdb2b5a/pdb
PDB entry 2b5a coloured by chain, front view.
PDB entry 2b5a coloured by chain, side view.
PDB entry 2b5a coloured by chain, top view.
Source organism: [Bacillus] caldolyticus
Primary publication:
Crystal structure of the restriction-modification system control element C.Bcll and mapping of its binding site.
Sawaya MR, Zhu Z, Mersha F, Chan SH, Dabur R, Xu SY, Balendiran GK
Structure 13 1837-47 (2005)
PMID: 16338412

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162642 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C.BclI Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 77 amino acids
Theoretical weight: 8.93 KDa
Source organism: [Bacillus] caldolyticus
Expression system: Escherichia coli
Structure domains: lambda repressor-like DNA-binding domains

Ligands and Environments

1 bound ligand:
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1, SSRL BEAMLINE BL1-5
Spacegroup: P21212
Unit cell:
a: 63.205Å b: 65.016Å c: 73.296Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.165 0.2
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Conflicts targeting epigenetic systems and their resolution by cell death: novel concepts for methyl-specific and other restriction systems.
Ishikawa et al. (2010)
1 more

6 mentions without citation

Structural analysis of the genetic switch that regulates the expression of restriction-modification genes.
McGeehan et al. (2008)
5 more