› 2b5e

X-ray diffraction
2.4Å resolution

Crystal Structure of Yeast Protein Disulfide Isomerase

Released:
DOI: 10.2210/pdb2b5e/pdb
PDB entry 2b5e coloured by chain, front view.
PDB entry 2b5e coloured by chain, side view.
PDB entry 2b5e coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structure of yeast protein disulfide isomerase suggests cooperativity between its active sites.
Tian G, Xiang S, Noiva R, Lennarz WJ, Schindelin H
Cell 124 61-73 (2006)
PMID: 16413482

Function and Biology Details

Reaction catalysed: 
Catalyzes the rearrangement of -S-S- bonds in proteins
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148228 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein disulfide-isomerase Chain: A
Molecule details ›
Chain: A
Length: 504 amino acids
Theoretical weight: 56.42 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P17967 (Residues: 23-522; Coverage: 100%)
Gene names: MFP1, PDI1, TRG1, YCL043C, YCL313, YCL43C
Sequence domains:
Structure domains: Glutaredoxin

Ligands and Environments

2 bound ligands:
Ligand BA 1 x BA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: I4
Unit cell:
a: 136.56Å b: 136.56Å c: 69.524Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.246
Expression system: Escherichia coli BL21

Quick links

Citations

72 review citations

In and out of the ER: protein folding, quality control, degradation, and related human diseases.
Hebert et al. (2007)
71 more

31 mentions without citation

Chaperones and foldases in endoplasmic reticulum stress signaling in plants.
Gupta et al. (2011)
30 more