› 2b5f

X-ray diffraction
3.9Å resolution

Crystal structure of the spinach aquaporin SoPIP2;1 in an open conformation to 3.9 resolution

Released:
DOI: 10.2210/pdb2b5f/pdb
PDB entry 2b5f coloured by chain, front view.
PDB entry 2b5f coloured by chain, side view.
PDB entry 2b5f coloured by chain, top view.
Source organism: Spinacia oleracea
Primary publication:
Structural mechanism of plant aquaporin gating.
T��rnroth-Horsefield S, Wang Y, Hedfalk K, Johanson U, Karlsson M, Tajkhorshid E, Neutze R, Kjellbom P
Nature 439 688-94 (2006)
PMID: 16340961

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aquaporin PIP-type Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 303 amino acids
Theoretical weight: 32.54 KDa
Source organism: Spinacia oleracea
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q41372 (Residues: 1-281; Coverage: 100%)
Gene name: LOC110800173
Sequence domains: Major intrinsic protein
Structure domains: Glycerol uptake facilitator protein.

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 181.3Å b: 103.97Å c: 67.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.31 0.29 0.332
Expression system: Komagataella pastoris

Quick links

Citations

106 review citations

Plant aquaporins: membrane channels with multiple integrated functions.
Maurel et al. (2008)
105 more

18 mentions without citation

The recombinant expression systems for structure determination of eukaryotic membrane proteins.
He et al. (2014)
17 more