› 2b5u

X-ray diffraction
2.3Å resolution

Crystal Structure Of Colicin E3 V206C Mutant In Complex With Its Immunity Protein

Released:
DOI: 10.2210/pdb2b5u/pdb
PDB entry 2b5u coloured by chain, front view.
PDB entry 2b5u coloured by chain, side view.
PDB entry 2b5u coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Nallini Vijayarangan A, Nithianantham S, Nan W, Jakes K, Shoham M

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132796 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Colicin E3 Chains: A, C
Molecule details ›
Chains: A, C
Length: 551 amino acids
Theoretical weight: 58.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli str. K-12 substr. W3110
UniProt:
  • Canonical: P00646 (Residues: 1-551; Coverage: 100%)
Gene names: ceaC, col
Sequence domains:
Structure domains:
Colicin E3 immunity protein Chains: B, D
Molecule details ›
Chains: B, D
Length: 84 amino acids
Theoretical weight: 9.78 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli str. K-12 substr. W3110
UniProt:
  • Canonical: P02984 (Residues: 2-85; Coverage: 99%)
Gene names: ceiC, imm, immB
Sequence domains: Cloacin immunity protein
Structure domains: Cloacin immunity protein

Ligands and Environments

1 bound ligand:
Ligand CIT 2 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21
Unit cell:
a: 66.73Å b: 192.916Å c: 85.385Å
α: 90° β: 112.98° γ: 90°
R-values:
R R work R free
0.295 0.259 0.295
Expression system: Escherichia coli str. K-12 substr. W3110

Quick links