› 2b6c

X-ray diffraction
2.1Å resolution

Predicted DNA alkylation repair enzyme from Enterococcus faecalis.

Released:
DOI: 10.2210/pdb2b6c/pdb
PDB entry 2b6c coloured by chain, front view.
PDB entry 2b6c coloured by chain, side view.
PDB entry 2b6c coloured by chain, top view.
Source organism: Enterococcus faecalis
Entry authors: Osipiuk J, Hatzos C, Moy S, Collart F, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182740 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-O-methylguanine DNA methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 26.14 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82ZI8 (Residues: 1-217; Coverage: 100%)
Gene name: EF_3068
Sequence domains: DNA alkylation repair enzyme
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 5 x SO4
Ligand CL 1 x CL
Ligand BME 2 x BME
Ligand GOL 4 x GOL
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P65
Unit cell:
a: 155.714Å b: 155.714Å c: 46.287Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.158 0.202
Expression system: Escherichia coli BL21(DE3)

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