› 2b8h

X-ray diffraction
2.2Å resolution

A/NWS/whale/Maine/1/84 (H1N9) reassortant influenza virus neuraminidase

Released:
DOI: 10.2210/pdb2b8h/pdb
PDB entry 2b8h coloured by chain, front view.
PDB entry 2b8h coloured by chain, side view.
PDB entry 2b8h coloured by chain, top view.
Source organism: Influenza A virus
Primary publication:
Structure of a calcium-deficient form of influenza virus neuraminidase: implications for substrate binding.
Smith BJ, Huyton T, Joosten RP, McKimm-Breschkin JL, Zhang JG, Luo CS, Lou MZ, Labrou NE, Garrett TP
Acta Crystallogr D Biol Crystallogr 62 947-52 (2006)
PMID: 16929094

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138781 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Neuraminidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 388 amino acids
Theoretical weight: 43.63 KDa
Source organism: Influenza A virus
Expression system: Gallus gallus
UniProt:
  • Canonical: P05803 (Residues: 83-470; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
4 bound ligands:
Ligand NAG 4 x NAG
Ligand SO4 29 x SO4
Ligand CL 4 x CL
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P3221
Unit cell:
a: 107.516Å b: 107.516Å c: 338.487Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.141 0.139 0.189
Expression system: Gallus gallus

Quick links

Citations

6 review citations

Influenza Virus Neuraminidase Structure and Functions.
McAuley et al. (2019)
5 more

4 mentions without citation

Function and 3D structure of the N-glycans on glycoproteins.
Nagae et al. (2012)
3 more