› 2b8i

X-ray diffraction
1.8Å resolution

Crystal Structure and Functional Studies Reveal that PAS Factor from Vibrio vulnificus is a Novel Member of the Saposin-Fold Family

Released:
DOI: 10.2210/pdb2b8i/pdb
PDB entry 2b8i coloured by chain, front view.
PDB entry 2b8i coloured by chain, side view.
PDB entry 2b8i coloured by chain, top view.
Source organism: Vibrio vulnificus
Primary publication:
Crystal structure and functional studies reveal that PAS factor from Vibrio vulnificus is a novel member of the saposin-fold family.
Lee JH, Yang ST, Rho SH, Im YJ, Kim SY, Kim YR, Kim MK, Kang GB, Kim JI, Rhee JH, Eom SH
J Mol Biol 355 491-500 (2006)
PMID: 16318855

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176391 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pas factor saposin domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 77 amino acids
Theoretical weight: 8.57 KDa
Source organism: Vibrio vulnificus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q56GA4 (Residues: 1-73; Coverage: 96%)
Gene name: PAS2
Sequence domains: Pas factor saposin fold
Structure domains: Pas factor, saposin domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: F222
Unit cell:
a: 57.157Å b: 75.192Å c: 80.206Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.232
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Synthetic surfactants with SP-B and SP-C analogues to enable worldwide treatment of neonatal respiratory distress syndrome and other lung diseases.
Johansson et al. (2019)
2 other articles

5 mentions without citation

Macromolecular ab initio phasing enforcing secondary and tertiary structure.
Millán et al. (2015)
4 more