› 2b9c

X-ray diffraction
2.3Å resolution

Structure of tropomyosin's mid-region: bending and binding sites for actin

Released:
DOI: 10.2210/pdb2b9c/pdb
PDB entry 2b9c coloured by chain, front view.
PDB entry 2b9c coloured by chain, side view.
PDB entry 2b9c coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure of the mid-region of tropomyosin: bending and binding sites for actin.
Brown JH, Zhou Z, Reshetnikova L, Robinson H, Yammani RD, Tobacman LS, Cohen C
Proc Natl Acad Sci U S A 102 18878-83 (2005)
PMID: 16365313

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tropomyosin alpha-1 chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 147 amino acids
Theoretical weight: 17.13 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04692 (Residues: 88-208; Coverage: 43%)
Gene names: Alpha-tm, Tpm1, Tpma
Sequence domains: Tropomyosin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1, NSLS BEAMLINE X29A
Spacegroup: P65
Unit cell:
a: 80.515Å b: 80.515Å c: 112.453Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.256 0.256 0.296
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Gestalt-binding of tropomyosin to actin filaments.
Holmes et al. (2008)
15 more

21 mentions without citation

A new twist on tropomyosin binding to actin filaments: perspectives on thin filament function, assembly and biomechanics.
Lehman et al. (2020)
20 more