› 2b9j

X-ray diffraction
2.3Å resolution

Crystal structure of Fus3 with a docking motif from Far1

Released:
DOI: 10.2210/pdb2b9j/pdb
PDB entry 2b9j coloured by chain, front view.
PDB entry 2b9j coloured by chain, side view.
PDB entry 2b9j coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The role of docking interactions in mediating signaling input, output, and discrimination in the yeast MAPK network.
Reményi A, Good MC, Bhattacharyya RP, Lim WA
Mol Cell 20 951-62 (2005)
PMID: 16364919

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147836 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitogen-activated protein kinase FUS3 Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 40.81 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P16892 (Residues: 1-353; Coverage: 100%)
Gene names: DAC2, FUS3, YBL016W, YBL03.21, YBL0303
Sequence domains: Protein kinase domain
Structure domains:
Cyclin-dependent kinase inhibitor FAR1 Chain: C
Molecule details ›
Chain: C
Length: 13 amino acids
Theoretical weight: 1.43 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 58.298Å b: 63.237Å c: 100.847Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.263
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

Mechanisms of specificity in protein phosphorylation.
Ubersax et al. (2007)
18 more

2 mentions without citation

Molecular basis of MAP kinase regulation.
Peti et al. (2013)
1 more