› 2ba0

X-ray diffraction
2.7Å resolution

Archaeal exosome core

Released:
DOI: 10.2210/pdb2ba0/pdb
PDB entry 2ba0 coloured by chain, front view.
PDB entry 2ba0 coloured by chain, side view.
PDB entry 2ba0 coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Structural framework for the mechanism of archaeal exosomes in RNA processing.
Büttner K, Wenig K, Hopfner KP
Mol Cell 20 461-71 (2005)
PMID: 16285927

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128161 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Exosome complex component Rrp4 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 229 amino acids
Theoretical weight: 25.59 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O29758 (Residues: 1-223; Coverage: 100%)
Gene names: AF_0492, rrp4
Sequence domains:
Structure domains:
Exosome complex component Rrp41 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 258 amino acids
Theoretical weight: 28.89 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O29757 (Residues: 1-258; Coverage: 100%)
Gene names: AF_0493, rrp41
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain
Exosome complex component Rrp42 Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 259 amino acids
Theoretical weight: 28.68 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O29756 (Residues: 1-259; Coverage: 100%)
Gene names: AF_0494, rrp42
Sequence domains:
Structure domains: GHMP Kinase, N-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 101.57Å b: 129.59Å c: 102.33Å
α: 90° β: 101.56° γ: 90°
R-values:
R R work R free
0.216 0.216 0.264
Expression system: Escherichia coli

Quick links

Citations

31 review citations

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
30 more

6 mentions without citation

Information available at cut rates: structure and mechanism of ribonucleases.
Worrall et al. (2007)
5 more