› 2bb5

X-ray diffraction
3.2Å resolution

Structure of Human Transcobalamin in complex with Cobalamin

Released:
DOI: 10.2210/pdb2bb5/pdb
PDB entry 2bb5 coloured by chain, front view.
PDB entry 2bb5 coloured by chain, side view.
PDB entry 2bb5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for mammalian vitamin B12 transport by transcobalamin.
Wuerges J, Garau G, Geremia S, Fedosov SN, Petersen TE, Randaccio L
Proc Natl Acad Sci U S A 103 4386-91 (2006)
PMID: 16537422

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcobalamin-2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 409 amino acids
Theoretical weight: 45.62 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P20062 (Residues: 19-427; Coverage: 100%)
Gene names: TC2, TCN2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand B12 2 x B12
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 49.127Å b: 145.497Å c: 165.043Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.258 0.257 0.288
Expression system: Komagataella pastoris

Quick links

Citations

17 review citations

Vitamin B12 transport from food to the body's cells--a sophisticated, multistep pathway.
Nielsen et al. (2012)
16 more

10 mentions without citation

Vitamin B12: unique metalorganic compounds and the most complex vitamins.
Randaccio et al. (2010)
9 more