› 2bcd

X-ray diffraction
2.1Å resolution

X-ray crystal structure of Protein Phosphatase-1 with the marine toxin motuporin bound

Released:
DOI: 10.2210/pdb2bcd/pdb
PDB entry 2bcd coloured by chain, front view.
PDB entry 2bcd coloured by chain, side view.
PDB entry 2bcd coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of protein phosphatase-1 bound to motuporin and dihydromicrocystin-LA: elucidation of the mechanism of enzyme inhibition by cyanobacterial toxins.
Maynes JT, Luu HA, Cherney MM, Andersen RJ, Williams D, Holmes CF, James MN
J Mol Biol 356 111-20 (2006)
PMID: 16343532

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153236 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 37.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P36873 (Residues: 1-323; Coverage: 100%)
Gene name: PPP1CC
Sequence domains:
Structure domains: Purple Acid Phosphatase; chain A, domain 2
MOTUPORIN Chain: B
Molecule details ›
Chain: B
Length: 5 amino acids
Theoretical weight: 786 Da
Source organism: Theonella sp.
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand BME 2 x BME
Ligand MN 8 x MN
3 modified residues:
Ligand ACB 1 x ACB
Ligand FGA 1 x FGA
Ligand MDH 1 x MDH

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P42212
Unit cell:
a: 100.955Å b: 100.955Å c: 63.485Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.264
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

15 review citations

Molecular mechanisms of microcystin toxicity in animal cells.
Campos et al. (2010)
14 more

6 mentions without citation

Structural basis for protein phosphatase 1 regulation and specificity.
Peti et al. (2013)
5 more