› 2bcj

X-ray diffraction
3.06Å resolution

Crystal Structure of G Protein-Coupled Receptor Kinase 2 in Complex with Galpha-q and Gbetagamma Subunits

Released:
DOI: 10.2210/pdb2bcj/pdb
PDB entry 2bcj coloured by chain, front view.
PDB entry 2bcj coloured by chain, side view.
PDB entry 2bcj coloured by chain, top view.
Source organisms:
Primary publication:
Snapshot of activated G proteins at the membrane: the Galphaq-GRK2-Gbetagamma complex.
Tesmer VM, Kawano T, Shankaranarayanan A, Kozasa T, Tesmer JJ
Science 310 1686-90 (2005)
PMID: 16339447

Function and Biology Details

Reaction catalysed: 
ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145465 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Beta-adrenergic receptor kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 689 amino acids
Theoretical weight: 79.75 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P21146 (Residues: 1-689; Coverage: 100%)
Gene names: ADRBK1, GRK2
Sequence domains:
Structure domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.31 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62871 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain
Guanine nucleotide-binding protein G(i) subunit alpha-1; Guanine nucleotide-binding protein G(q) subunit alpha Chain: Q
Molecule details ›
Chain: Q
Length: 353 amino acids
Theoretical weight: 41.3 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P21279 (Residues: 7-7, 37-359; Coverage: 90%)
  • Canonical: P10824 (Residues: 2-28; Coverage: 8%)
Gene names: Gnai-1, Gnai1, Gnaq
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand ALF 1 x ALF
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 64.918Å b: 129.954Å c: 122.764Å
α: 90° β: 95.81° γ: 90°
R-values:
R R work R free
0.236 0.243 0.292
Expression system: Trichoplusia ni

Quick links

Citations

55 review citations

The nuts and bolts of AGC protein kinases.
Pearce et al. (2010)
54 more

23 mentions without citation

Discovery of new GPCR biology: one receptor structure at a time.
Hanson et al. (2009)
22 more