› 2bco

X-ray diffraction
2.33Å resolution

X-ray structure of succinylglutamate desuccinalase from Vibrio Parahaemolyticus (RIMD 2210633) at the resolution 2.3 A, Northeast Structural Genomics Target Vpr14

Released:
DOI: 10.2210/pdb2bco/pdb
PDB entry 2bco coloured by chain, front view.
PDB entry 2bco coloured by chain, side view.
PDB entry 2bco coloured by chain, top view.
Source organism: Vibrio parahaemolyticus
Entry authors: Kuzin AP, Abashidze M, Forouhar F, Benach J, Zhou W, Acton T, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
N-succinyl-L-glutamate + H(2)O = succinate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183361 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Succinylglutamate desuccinylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 350 amino acids
Theoretical weight: 40.24 KDa
Source organism: Vibrio parahaemolyticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q87Q40 (Residues: 1-342; Coverage: 100%)
Gene names: VP1310, astE
Sequence domains: Succinylglutamate desuccinylase / Aspartoacylase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 62.852Å b: 72.555Å c: 93.032Å
α: 90° β: 105.66° γ: 90°
R-values:
R R work R free
0.213 0.213 0.267
Expression system: Escherichia coli

Quick links