› 2bd0

X-ray diffraction
1.7Å resolution

Chlorobium tepidum Sepiapterin Reductase complexed with NADP and Sepiapterin

Released:
DOI: 10.2210/pdb2bd0/pdb
PDB entry 2bd0 coloured by chain, front view.
PDB entry 2bd0 coloured by chain, side view.
PDB entry 2bd0 coloured by chain, top view.
Source organism: Chlorobaculum tepidum TLS
Primary publication:
Structure of Chlorobium tepidum sepiapterin reductase complex reveals the novel substrate binding mode for stereospecific production of L-threo-tetrahydrobiopterin.
Supangat S, Seo KH, Choi YK, Park YS, Son D, Han CD, Lee KH
J Biol Chem 281 2249-56 (2006)
PMID: 16308317

Function and Biology Details

Reaction catalysed: 
L-threo-7,8-dihydrobiopterin + NADP(+) = sepiapterin + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185123 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sepiapterin reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 244 amino acids
Theoretical weight: 27.15 KDa
Source organism: Chlorobaculum tepidum TLS
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KES3 (Residues: 1-244; Coverage: 100%)
Gene name: CT0609
Sequence domains: short chain dehydrogenase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand NAP 4 x NAP
Ligand BIO 1 x BIO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B, PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 84.359Å b: 97.482Å c: 123.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.213
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

Folate salvage in plants: pterin aldehyde reduction is mediated by multiple non-specific aldehyde reductases.
Noiriel et al. (2007)
5 more

3 mentions without citation

Sepiapterin reductase: Characteristics and role in diseases.
Wu et al. (2020)
2 more