› 2bdt

X-ray diffraction
2.4Å resolution

Crystal Structure of the Putative Gluconate Kinase from Bacillus halodurans, Northeast Structural Genomics Target BhR61

Released:
DOI: 10.2210/pdb2bdt/pdb
PDB entry 2bdt coloured by chain, front view.
PDB entry 2bdt coloured by chain, side view.
PDB entry 2bdt coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Entry authors: Forouhar F, Abashidze M, Jayaraman S, Janjua H, Cooper B, Xiao R, Acton TB, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
ATP + D-gluconate = ADP + 6-phospho-D-gluconate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
gluconokinase Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 22.01 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9K6P2 (Residues: 1-176; Coverage: 100%)
Gene name: BH3686
Sequence domains: AAA domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P43212
Unit cell:
a: 43.675Å b: 43.675Å c: 198.846Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.233 0.287
Expression system: Escherichia coli

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