› 2bek

X-ray diffraction
1.8Å resolution

Structure of the bacterial chromosome segregation protein Soj

Released:
DOI: 10.2210/pdb2bek/pdb
PDB entry 2bek coloured by chain, front view.
PDB entry 2bek coloured by chain, side view.
PDB entry 2bek coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
Bacterial chromosome segregation: structure and DNA binding of the Soj dimer--a conserved biological switch.
Leonard TA, Butler PJ, Löwe J
EMBO J 24 270-82 (2005)
PMID: 15635448

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180819 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chromosome-partitioning ATPase Soj Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 257 amino acids
Theoretical weight: 27.52 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72H90 (Residues: 1-249; Coverage: 100%)
Gene names: TT_C1605, soj
Sequence domains: AAA domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ATP 4 x ATP
Ligand MG 4 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 87.434Å b: 95.908Å c: 123.943Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.216
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

39 review citations

Assembly dynamics of the bacterial MinCDE system and spatial regulation of the Z ring.
Lutkenhaus J. (2007)
38 more

15 mentions without citation

Bacterial chromosome segregation: structure and DNA binding of the Soj dimer--a conserved biological switch.
Leonard et al. (2005)
14 more