› 2beo

X-ray diffraction
2.7Å resolution

PrfA, Transcriptional Regulator In Listeria Monocytogenes

Released:
DOI: 10.2210/pdb2beo/pdb
PDB entry 2beo coloured by chain, front view.
PDB entry 2beo coloured by chain, side view.
PDB entry 2beo coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Primary publication:
The mutation G145S in PrfA, a key virulence regulator of Listeria monocytogenes, increases DNA-binding affinity by stabilizing the HTH motif.
Eiting M, Hagelüken G, Schubert WD, Heinz DW
Mol Microbiol 56 433-46 (2005)
PMID: 15813735

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Listeriolysin regulatory protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 236 amino acids
Theoretical weight: 27.2 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli
UniProt:
  • Canonical: P22262 (Residues: 2-237; Coverage: 100%)
Gene names: lmo0200, prfA
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GLN 1 x GLN
Ligand CL 2 x CL
Ligand PG4 1 x PG4
Ligand ACM 1 x ACM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 55.951Å b: 80.713Å c: 60.965Å
α: 90° β: 111.91° γ: 90°
R-values:
R R work R free
0.246 0.244 0.289
Expression system: Escherichia coli

Quick links

Citations

11 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
10 more

6 mentions without citation

The metabolic regulator CodY links Listeria monocytogenes metabolism to virulence by directly activating the virulence regulatory gene prfA.
Lobel et al. (2015)
5 more