› 2bf0

X-ray diffraction
2.3Å resolution

crystal structure of the rpr of pcf11

Released:
DOI: 10.2210/pdb2bf0/pdb
PDB entry 2bf0 coloured by chain, front view.
PDB entry 2bf0 coloured by chain, side view.
PDB entry 2bf0 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Key features of the interaction between Pcf11 CID and RNA polymerase II CTD.
Noble CG, Hollingworth D, Martin SR, Ennis-Adeniran V, Smerdon SJ, Kelly G, Taylor IA, Ramos A
Nat Struct Mol Biol 12 144-51 (2005)
PMID: 15665873

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153821 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein PCF11 Chain: X
Molecule details ›
Chain: X
Length: 143 amino acids
Theoretical weight: 16.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P39081 (Residues: 1-138; Coverage: 22%)
Gene names: PCF11, YD9934.13C, YDR228C
Sequence domains: CID domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P43212
Unit cell:
a: 75.494Å b: 75.494Å c: 56.135Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.258 0.282
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

Rules of engagement: co-transcriptional recruitment of pre-mRNA processing factors.
Bentley DL. (2005)
22 more

3 mentions without citation

Structural biology of poly(A) site definition.
Yang et al. (2011)
2 more