› 2bgc

X-ray diffraction
2.3Å resolution

PrfA-G145S, a constitutive active mutant of the Transcriptional Regulator In L.monocytogenes

Released:
DOI: 10.2210/pdb2bgc/pdb
PDB entry 2bgc coloured by chain, front view.
PDB entry 2bgc coloured by chain, side view.
PDB entry 2bgc coloured by chain, top view.
Source organism: Listeria monocytogenes EGD-e
Primary publication:
The mutation G145S in PrfA, a key virulence regulator of Listeria monocytogenes, increases DNA-binding affinity by stabilizing the HTH motif.
Eiting M, Hagelüken G, Schubert WD, Heinz DW
Mol Microbiol 56 433-46 (2005)
PMID: 15813735

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Listeriolysin regulatory protein Chains: A, B, D, E, F, G, H, I
Molecule details ›
Chains: A, B, D, E, F, G, H, I
Length: 238 amino acids
Theoretical weight: 27.43 KDa
Source organism: Listeria monocytogenes EGD-e
Expression system: Escherichia coli
UniProt:
  • Canonical: P22262 (Residues: 2-237; Coverage: 100%)
Gene names: lmo0200, prfA
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand DTU 1 x DTU
Ligand DTT 1 x DTT
1 modified residue:
Ligand PR3 8 x PR3

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: C2
Unit cell:
a: 117.247Å b: 100.243Å c: 189.563Å
α: 90° β: 90.33° γ: 90°
R-values:
R R work R free
0.199 0.195 0.268
Expression system: Escherichia coli

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Citations

11 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
10 more

4 mentions without citation

Structural basis for glutathione-mediated activation of the virulence regulatory protein PrfA in Listeria.
Hall et al. (2016)
3 more