› 2bgg

X-ray diffraction
2.2Å resolution

The structure of a Piwi protein from Archaeoglobus fulgidus complexed with a 16nt siRNA duplex.

Released:
DOI: 10.2210/pdb2bgg/pdb
PDB entry 2bgg coloured by chain, front view.
PDB entry 2bgg coloured by chain, side view.
PDB entry 2bgg coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Structural insights into mRNA recognition from a PIWI domain-siRNA guide complex.
Parker JS, Roe SM, Barford D
Nature 434 663-6 (2005)
PMID: 15800628

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128086 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Piwi protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 427 amino acids
Theoretical weight: 49.3 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28951 (Residues: 1-427; Coverage: 100%)
Gene name: AF_1318
Sequence domains: Piwi domain
Structure domains:
5'-R(*UP*UP*CP*GP*AP*CP*GP*CP)-3' Chains: P, R
Molecule details ›
Chains: P, R
Length: 8 nucleotides
Theoretical weight: 2.5 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided
5'-R(*GP*UP*CP*GP*AP*AP*UP*UP)-3' Chains: Q, S
Molecule details ›
Chains: Q, S
Length: 8 nucleotides
Theoretical weight: 2.53 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P1
Unit cell:
a: 51.916Å b: 61.206Å c: 104.059Å
α: 76.54° β: 76.14° γ: 79.35°
R-values:
R R work R free
0.183 0.18 0.243
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

108 review citations

MicroRNAs: target recognition and regulatory functions.
Bartel DP. (2009)
107 more

18 mentions without citation

Structural domains in RNAi.
Collins et al. (2005)
17 more