› 2bgi

X-ray diffraction
1.68Å resolution

X-Ray Structure of the Ferredoxin-NADP(H) Reductase from Rhodobacter capsulatus complexed with three molecules of the detergent n-heptyl- beta-D-thioglucoside at 1.7 Angstroms

Released:
DOI: 10.2210/pdb2bgi/pdb
PDB entry 2bgi coloured by chain, front view.
PDB entry 2bgi coloured by chain, side view.
PDB entry 2bgi coloured by chain, top view.
Source organism: Rhodobacter capsulatus
Primary publication:
The ferredoxin-NADP(H) reductase from Rhodobacter capsulatus: molecular structure and catalytic mechanism.
Nogués I, Pérez-Dorado I, Frago S, Bittel C, Mayhew SG, Gómez-Moreno C, Hermoso JA, Medina M, Cortez N, Carrillo N
Biochemistry 44 11730-40 (2005)
PMID: 16128574

Function and Biology Details

Reactions catalysed: 
2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH
Reduced flavodoxin + NADP(+) = oxidized flavodoxin + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192220 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavodoxin/ferredoxin--NADP reductase Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 30.44 KDa
Source organism: Rhodobacter capsulatus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9L6V3 (Residues: 1-270; Coverage: 100%)
Gene name: fpr
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
3 bound ligands:
Ligand HTG 3 x HTG
Ligand SO4 1 x SO4
Ligand CO2 2 x CO2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P3121
Unit cell:
a: 120.848Å b: 120.848Å c: 50.709Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.221 0.225
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Structural and functional diversity of ferredoxin-NADP(+) reductases.
Aliverti et al. (2008)
3 more

1 mention without citation

Identification of Inhibitors Targeting Ferredoxin-NADP⁺ Reductase from the Xanthomonas citri subsp. citri Phytopathogenic Bacteria.
Martínez-Júlvez et al. (2017)