› 2bh9

X-ray diffraction
2.5Å resolution

X-RAY STRUCTURE OF A DELETION VARIANT OF HUMAN GLUCOSE 6-PHOSPHATE DEHYDROGENASE COMPLEXED WITH STRUCTURAL AND COENZYME NADP

Released:
DOI: 10.2210/pdb2bh9/pdb
PDB entry 2bh9 coloured by chain, front view.
PDB entry 2bh9 coloured by chain, side view.
PDB entry 2bh9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural studies of glucose-6-phosphate and NADP+ binding to human glucose-6-phosphate dehydrogenase.
Kotaka M, Gover S, Vandeputte-Rutten L, Au SW, Lam VM, Adams MJ
Acta Crystallogr D Biol Crystallogr 61 495-504 (2005)
PMID: 15858258

Function and Biology Details

Reaction catalysed: 
D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145820 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate 1-dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 489 amino acids
Theoretical weight: 56.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11413 (Residues: 27-515; Coverage: 95%)
Gene name: G6PD
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: F222
Unit cell:
a: 60.76Å b: 172.48Å c: 217.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.196 0.296
Expression system: Escherichia coli

Quick links

Citations

7 review citations

G6PD deficiency: the genotype-phenotype association.
Mason et al. (2007)
6 more

47 mentions without citation

Glucose-6-Phosphate Dehydrogenase: Update and Analysis of New Mutations around the World.
Gómez-Manzo et al. (2016)
46 more