› 2bi0

X-ray diffraction
1.9Å resolution

RV0216, A conserved hypothetical protein from Mycobacterium tuberculosis that is essential for bacterial survival during infection, has a double hotdogfold

Released:
DOI: 10.2210/pdb2bi0/pdb
PDB entry 2bi0 coloured by chain, front view.
PDB entry 2bi0 coloured by chain, side view.
PDB entry 2bi0 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Rv0216, a conserved hypothetical protein from Mycobacterium tuberculosis that is essential for bacterial survival during infection, has a double hotdog fold.
Castell A, Johansson P, Unge T, Jones TA, Bäckbro K
Protein Sci 14 1850-62 (2005)
PMID: 15987908

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161217 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acyl dehydratase Chain: A
Molecule details ›
Chain: A
Length: 337 amino acids
Theoretical weight: 35.94 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P96398 (Residues: 1-337; Coverage: 100%)
Gene name: MT0226
Structure domains: Hotdog Thioesterase

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 3 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6322
Unit cell:
a: 78.75Å b: 78.75Å c: 180.309Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.173 0.217
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Targeting the formation of the cell wall core of M. tuberculosis.
Barry et al. (2007)
4 more

5 mentions without citation

Analysis of proteins with the 'hot dog' fold: prediction of function and identification of catalytic residues of hypothetical proteins.
Pidugu et al. (2009)
4 more