› 2bis

X-ray diffraction
2.8Å resolution

Structure of glycogen synthase from Pyrococcus abyssi

Released:
DOI: 10.2210/pdb2bis/pdb
PDB entry 2bis coloured by chain, front view.
PDB entry 2bis coloured by chain, side view.
PDB entry 2bis coloured by chain, top view.
Source organism: Pyrococcus abyssi
Primary publication:
Crystal structure of an archaeal glycogen synthase: insights into oligomerization and substrate binding of eukaryotic glycogen synthases.
Horcajada C, Guinovart JJ, Fita I, Ferrer JC
J Biol Chem 281 2923-31 (2006)
PMID: 16319074

Function and Biology Details

Reaction catalysed: 
ADP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = ADP + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194178 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Starch synthase catalytic domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 440 amino acids
Theoretical weight: 49.33 KDa
Source organism: Pyrococcus abyssi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9V2J8 (Residues: 1-437; Coverage: 100%)
Gene name: PAB2292
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

4 bound ligands:
Ligand GLC 2 x GLC
Ligand DIO 10 x DIO
Ligand GOL 7 x GOL
Ligand UDP 1 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 202.985Å b: 73.973Å c: 148.221Å
α: 90° β: 131.19° γ: 90°
R-values:
R R work R free
0.204 0.2 0.266
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
7 more

4 mentions without citation

Organochlorine pesticides, their toxic effects on living organisms and their fate in the environment.
Jayaraj et al. (2016)
3 more