› 2bj4

X-ray diffraction
2Å resolution

ESTROGEN RECEPTOR ALPHA LBD IN COMPLEX WITH A PHAGE-DISPLAY DERIVED PEPTIDE ANTAGONIST

Released:
DOI: 10.2210/pdb2bj4/pdb
PDB entry 2bj4 coloured by chain, front view.
PDB entry 2bj4 coloured by chain, side view.
PDB entry 2bj4 coloured by chain, top view.
Source organisms:
Primary publication:
Delineation of a unique protein-protein interaction site on the surface of the estrogen receptor.
Kong EH, Heldring N, Gustafsson JA, Treuter E, Hubbard RE, Pike AC
Proc Natl Acad Sci U S A 102 3593-8 (2005)
PMID: 15728727

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136905 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Estrogen receptor Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 28.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03372 (Residues: 303-533; Coverage: 39%)
Gene names: ESR, ESR1, NR3A1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Estrogen receptor Chain: B
Molecule details ›
Chain: B
Length: 252 amino acids
Theoretical weight: 28.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03372 (Residues: 303-533; Coverage: 39%)
Gene names: ESR, ESR1, NR3A1
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
PEPTIDE ANTAGONIST Chains: C, D
Molecule details ›
Chains: C, D
Length: 11 amino acids
Theoretical weight: 1.3 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand OHT 2 x OHT
1 modified residue:
Ligand CCS 1 x CCS

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 50.76Å b: 98.76Å c: 105.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.219
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

Estrogen receptors: how do they signal and what are their targets.
Heldring et al. (2007)
9 more

13 mentions without citation

Integrase, LEDGF/p75 and HIV replication.
Poeschla EM. (2008)
12 more