› 2bjh

X-ray diffraction
2.54Å resolution

Crystal Structure Of S133A AnFaeA-ferulic acid complex

Released:
DOI: 10.2210/pdb2bjh/pdb
PDB entry 2bjh coloured by chain, front view.
PDB entry 2bjh coloured by chain, side view.
PDB entry 2bjh coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
Probing the determinants of substrate specificity of a feruloyl esterase, AnFaeA, from Aspergillus niger.
Faulds CB, Molina R, Gonzalez R, Husband F, Juge N, Sanz-Aparicio J, Hermoso JA
FEBS J 272 4362-71 (2005)
PMID: 16128806

Function and Biology Details

Reaction catalysed: 
Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Feruloyl esterase A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 260 amino acids
Theoretical weight: 28.34 KDa
Source organism: Aspergillus niger
Expression system: Komagataella pastoris
UniProt:
  • Canonical: O42807 (Residues: 22-281; Coverage: 100%)
Gene name: faeA
Sequence domains: Lipase (class 3)
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand NAG 3 x NAG
Ligand NDG 3 x NDG
Ligand FER 3 x FER
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 46.75Å b: 130.75Å c: 76.5Å
α: 90° β: 98.14° γ: 90°
R-values:
R R work R free
0.216 0.216 0.277
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

Feruloyl esterases as biotechnological tools: current and future perspectives.
Fazary et al. (2007)
1 more

1 mention without citation

An inserted α/β subdomain shapes the catalytic pocket of Lactobacillus johnsonii cinnamoyl esterase.
Lai et al. (2011)