› 2bjm

X-ray diffraction
2.15Å resolution

SPE7:Anthrone Complex

Released:
DOI: 10.2210/pdb2bjm/pdb
PDB entry 2bjm coloured by chain, front view.
PDB entry 2bjm coloured by chain, side view.
PDB entry 2bjm coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure and kinetics of a transient antibody binding intermediate reveal a kinetic discrimination mechanism in antigen recognition.
James LC, Tawfik DS
Proc Natl Acad Sci U S A 102 12730-5 (2005)
PMID: 16129832

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208479 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
IGE SPE7 HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 120 amino acids
Theoretical weight: 13.55 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Ig lambda-1 chain V regions MOPC 104E/RPC20/J558/S104 Chain: L
Molecule details ›
Chain: L
Length: 110 amino acids
Theoretical weight: 11.56 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01724 (Residues: 20-128; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand ANF 1 x ANF
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I4
Unit cell:
a: 79.672Å b: 79.672Å c: 67.958Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.268 0.286
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Enzyme promiscuity: a mechanistic and evolutionary perspective.
Khersonsky et al. (2010)
13 more

1 mention without citation

Structure and kinetics of a transient antibody binding intermediate reveal a kinetic discrimination mechanism in antigen recognition.
James et al. (2005)