› 2bkg

X-ray diffraction
1.9Å resolution

Crystal structure of E3_19 a designed ankyrin repeat protein

Released:
DOI: 10.2210/pdb2bkg/pdb
PDB entry 2bkg coloured by chain, front view.
PDB entry 2bkg coloured by chain, side view.
PDB entry 2bkg coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Crystal structure of a consensus-designed ankyrin repeat protein: implications for stability.
Binz HK, Kohl A, Plückthun A, Grütter MG
Proteins 65 280-4 (2006)
PMID: 16493627

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162649 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SYNTHETIC CONSTRUCT ANKYRIN REPEAT PROTEIN E3_19 Chains: A, B
Molecule details ›
Chains: A, B
Length: 166 amino acids
Theoretical weight: 17.8 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 49.579Å b: 72.67Å c: 83.325Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.227
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy.
Plückthun A. (2015)
2 more

7 mentions without citation

A primer on ankyrin repeat function in TRP channels and beyond.
Gaudet R. (2008)
6 more