› 2bll

X-ray diffraction
2.3Å resolution

Apo-structure of the C-terminal decarboxylase domain of ArnA

Released:
DOI: 10.2210/pdb2bll/pdb
PDB entry 2bll coloured by chain, front view.
PDB entry 2bll coloured by chain, side view.
PDB entry 2bll coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure and function of both domains of ArnA, a dual function decarboxylase and a formyltransferase, involved in 4-amino-4-deoxy-L-arabinose biosynthesis.
Williams GJ, Breazeale SD, Raetz CR, Naismith JH
J Biol Chem 280 23000-8 (2005)
PMID: 15809294

Function and Biology Details

Reactions catalysed: 
UDP-alpha-D-glucuronate + NAD(+) = UDP-beta-L-threo-pentapyranos-4-ulose + CO(2) + NADH
10-formyltetrahydrofolate + UDP-4-amino-4-deoxy-beta-L-arabinose = 5,6,7,8-tetrahydrofolate + UDP-4-deoxy-4-formamido-beta-L-arabinose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160161 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional polymyxin resistance protein ArnA Chain: A
Molecule details ›
Chain: A
Length: 345 amino acids
Theoretical weight: 40.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77398 (Residues: 317-660; Coverage: 52%)
Gene names: JW2249, arnA, b2255, pmrI, yfbG
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P4132
Unit cell:
a: 149.438Å b: 149.438Å c: 149.438Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Lipid A modification systems in gram-negative bacteria.
Raetz et al. (2007)
7 more

3 mentions without citation

Structure and function of GDP-mannose-3',5'-epimerase: an enzyme which performs three chemical reactions at the same active site.
Major et al. (2005)
2 more