› 2bmi

X-ray diffraction
2Å resolution

METALLO-BETA-LACTAMASE

Released:
DOI: 10.2210/pdb2bmi/pdb
PDB entry 2bmi coloured by chain, front view.
PDB entry 2bmi coloured by chain, side view.
PDB entry 2bmi coloured by chain, top view.
Source organism: Bacteroides fragilis
Primary publication:
X-ray structure of the ZnII beta-lactamase from Bacteroides fragilis in an orthorhombic crystal form.
Carfi A, Duée E, Paul-Soto R, Galleni M, Frère JM, Dideberg O
Acta Crystallogr D Biol Crystallogr 54 45-57 (1998)
PMID: 9761816

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150610 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 25.36 KDa
Source organism: Bacteroides fragilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P25910 (Residues: 18-249; Coverage: 100%)
Gene names: ccrA, cfiA
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 47.256Å b: 94.92Å c: 111.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.262
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

Metallo-beta-lactamases: the quiet before the storm?
Walsh et al. (2005)
6 more

6 mentions without citation

Metallo-β-lactamase structure and function.
Palzkill T. (2013)
5 more